G(Z) COUPLING TO THE RAT KAPPA-OPIOID RECEPTOR

被引：58

作者：

LAI, HWL

MINAMI, M

SATOH, M

WONG, YH

机构：

[1] HONG KONG UNIV SCI & TECHNOL,DEPT BIOL,KOWLOON,HONG KONG

[2] KYOTO UNIV,FAC PHARMACEUT SCI,DEPT PHARMACOL,KYOTO 60601,JAPAN

[3] KYOTO UNIV,FAC PHARMACEUT SCI,DEPT MOLEC PHARMACOL,KYOTO 60601,JAPAN

来源：

FEBS LETTERS | 1995年 / 360卷 / 01期

关键词：

KAPPA-OPIOID RECEPTOR; ADENYLYL CYCLASE; G PROTEIN; SIGNAL TRANSDUCTION;

D O I：

10.1016/0014-5793(95)00088-Q

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have expressed the cloned rat kappa-opioid receptor in human embryonic kidney 293 cells and studied the ability of kappa-selective ligands to inhibit adenylyl cyclase, In transfected 293 cells, activation of the kappa-opioid receptor by U50,488 and the dynorphins resulted in the inhibition of cAMP accumulation. The inhibitory response was sensitive to pertussis toxin and highly selective for kappa-agonists; neither mu- nor delta-opioids were able to activate the kappa-opioid receptor, Upon co-transfection with the alpha subunit of G(z), inhibition of cAMP accumulation by kappa-agonist became refractory to pertussis toxin, indicating that the kappa-opioid receptor can couple to both G(i) and G(z) proteins.

引用

页码：97 / 99

页数：3

共 24 条

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