FLUORESCENCE ENERGY-TRANSFER FROM FLUORESCEIN TO TETRAMETHYLRHODAMINE COVALENTLY BOUND TO THE SURFACE OF POLYSTYRENE LATEX-PARTICLES

被引：23

作者：

CHARREYRE, MT

YEKTA, A

WINNIK, MA

DELAIR, T

PICHOT, C

机构：

[1] UNIV TORONTO,DEPT CHEM,TORONTO,ON M5S 1A1,CANADA

[2] UNIV TORONTO,ERINDALE COLL,TORONTO,ON M5S 1A1,CANADA

[3] ECOLE NORMALE SUPER LYON,CNRS BIOMERIEUX,UNITE MIXTE,F-69364 LYON,FRANCE

来源：

LANGMUIR | 1995年 / 11卷 / 07期

关键词：

D O I：

10.1021/la00007a019

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Steady-state fluorescence energy transfer experiments have been carried out with fluorescein (donor) and tetramethylrhodamine (acceptor) covalently bound onto the surface of amino functionalized polystyrene latex microspheres. 5-Carboxyfluorescein-NHS and 6-carboxytetramethylrhodamine-NHS ester were used to synthesize donor-labeled particles and acceptor-labeled particles respectively, as well as donor-acceptor-labeled particles. Some changes in the spectroscopic characteristics of the dyes after their covalent binding onto the PS latex were observed. A theoretical model describing a random distribution of dyes onto the surface of a sphere corroborates well the experimental energy transfer results.

引用

页码：2423 / 2428

页数：6

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