ELECTROPHORESIS OF URONIC-ACIDS, NEUTRAL SUGARS AND HYDROLYSATES OF ACIDIC POLYSACCHARIDES ON SILYLATED GLASS-FIBER PAPER IN ELECTROLYTES OF BIVALENT-CATIONS

The separation of different uronic acids, oligogalacturonic acids and neutral sugars by electrophoresis on silylated glass-fibre paper is described. As electrolytes 0.1 M solutions of barium, zinc and calcium acetate were used. In these solutions the separation of acid constituents was possible without interference from neutral sugars. Depending on the electrolytes, a mixture of galacturonic, mannuronic, glucuronic and guluronic acid was separated within 90-165 min. The partial characterization of acid polysaccharides such as pectin, alginate and mixtures thereof is described. © 1990.