INCREASE IN THE ATP SIGNAL AFTER TREATMENT WITH CISPLATIN IN 2 DIFFERENT CELL-LINES STUDIED BY P-31 NMR-SPECTROSCOPY

被引:18
作者
BERGHMANS, K
RUIZCABELLO, J
SIMPKINS, H
ANDREWS, PA
COHEN, JS
机构
[1] GEORGETOWN UNIV, SCH MED, DEPT PHARMACOL, 4 RES COURT, ROCKVILLE, MD 20850 USA
[2] SUNY DOWNSTATE, DEPT PATHOL, NEW YORK, NY USA
[3] STATEN ISL UNIV HOSP, NEW YORK, NY USA
关键词
D O I
10.1016/0006-291X(92)91616-X
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We have compared the 31P nuclear magnetic resonance (NMR) spectra of two different cisplatin resistant cell lines, one derived from human ovarian carcinoma and the other from rat lymphoma, and their respective cisplatin sensitive parental cells lines. Comparisons were made between the baseline spectra and after perfusion of the cells with 20-50 μM cisplatin for 16-20 hours. While no obvious differences were found between baseline spectra of sensitive and resistant cells, during cisplatin perfusion the sensitive cells had an increase in their ATP signals. The resistant cells also exhibited increases in their ATP signals during cisplatin perfusion but to a lesser extent than the sensitive cells. Although the significance of these ATP elevations towards the cellular pharmacology of cisplatin are not presently known, our studies demonstrate that 31P NMR spectroscopy may be useful for elucidating differences in phosphate metabolism in cells expressing the cisplatin resistant phenotype. © 1992.
引用
收藏
页码:114 / 120
页数:7
相关论文
共 22 条
[1]  
ANDREWS P A, 1990, Proceedings of the American Association for Cancer Research Annual Meeting, V31, P378
[2]  
ANDREWS PA, 1991, CANCER COMMUN, V3, P1
[3]  
COHEN JS, 1989, METHOD ENZYMOL, V177, P435
[4]   MULTINUCLEAR NMR-STUDY OF THE METABOLISM OF DRUG-SENSITIVE AND DRUG-RESISTANT HUMAN-BREAST CANCER-CELLS [J].
COHEN, JS ;
LYON, RC .
ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, 1987, 508 :216-228
[5]  
DALY PF, 1990, CANCER RES, V50, P552
[6]   CONTINUOUS PERFUSION OF MAMMALIAN-CELLS EMBEDDED IN AGAROSE-GEL THREADS [J].
FOXALL, DL ;
COHEN, JS ;
MITCHELL, JB .
EXPERIMENTAL CELL RESEARCH, 1984, 154 (02) :521-529
[7]   INFORMATION FROM COMBINED H-1 AND P-31 NMR-STUDIES OF CELL-EXTRACTS - DIFFERENCES IN METABOLISM BETWEEN DRUG-SENSITIVE AND DRUG-RESISTANT MCF-7 HUMAN BREAST-CANCER CELLS [J].
KAPLAN, O ;
VANZIJL, PCM ;
COHEN, JS .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1990, 169 (02) :383-390
[8]  
KAPLAN O, 1991, CANCER RES, V51, P1638
[9]   MODULATION OF CIS-DIAMMINEDICHLOROPLATINUM(II) ACCUMULATION AND SENSITIVITY BY FORSKOLIN AND 3-ISOBUTYL-1-METHYLXANTHINE IN SENSITIVE AND RESISTANT HUMAN OVARIAN-CARCINOMA CELLS [J].
MANN, SC ;
ANDREWS, PA ;
HOWELL, SB .
INTERNATIONAL JOURNAL OF CANCER, 1991, 48 (06) :866-872
[10]   MULTIDRUG RESISTANCE [J].
MOSCOW, JA ;
COWAN, KH .
JOURNAL OF THE NATIONAL CANCER INSTITUTE, 1988, 80 (01) :14-20