ANTIGENIC ANALYSES OF SUGI BASIC-PROTEIN BY MONOCLONAL-ANTIBODIES .2. DETECTION OF IMMUNOREACTIVE FRAGMENTS IN ENZYME-CLEAVED CRY-J-I

被引:19
作者
KAWASHIMA, T
TANIAI, M
USUI, M
ANDO, S
KURIMOTO, M
MATUHASI, T
机构
[1] HAYASHIBARA BIOCHEM LABS INC,675-1 FUJISAKI,OKAYAMA 702,JAPAN
[2] OKINAKA MEM INST MED RES,TOKYO,JAPAN
关键词
CRY-J-I; EPITOPE; AMINO ACID SEQUENCE; ENZYMATIC CLEAVAGE; SYNTHETIC PEPTIDE; MONOCLONAL ANTIBODIES;
D O I
10.1159/000236174
中图分类号
R392 [医学免疫学];
学科分类号
100102 ;
摘要
The 4 anti-Cry j I mAbs showing an epitope specificity different from each other, 046, 029, 026 and 027, were selected to analyze the structure of the antigenic determinant for each mAb on a Cry j I molecule. Immunoreactive fragments in enzyme-cleaved Cry j I were detected by means of the adsorption on the mAb column and of the binding to the mAbs on Elisa. The mAb 026 was found to be reactive to the fragments containing a Cry j I N-terminal region obtained by V8 protease or pepsin digestion, but not to those by lysylendopeptidase digestion. The mAb 027 was found to be capable of binding to the fragments containing a linear structure of Asn-Ala-Gly-Val-Leu-Thr-Cys-Ser-Leu-Ser-Lys, which were generated by V8 protease, lysylendopeptidase or pepsin digestion. Furthermore, the synthetic peptide Asn-Ala-Gly-Val-Leu-Thr-Cys-Ser-Leu-Ser-Lys-Arg could bind to 027, but not to 026, and could inhibit the binding of 027 to Cry j I or to its immunoreactive fragments. No fragments capable of reacting to the mAbs 046 and 029 could be found in this study, suggesting that 046 and 029 recognize a conformationally constituted epitope of Cry j I molecule which is destroyed by enzymatic cleavage. The epitope recognized by the mAbs 027 or 026 was found to be located in conformationally hidden parts of the molecule which was exposed to react to the mAbs only after the physicochemical or enzymatic treatment.
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页码:118 / 126
页数:9
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