ALTERATIONS OF THE GLYCAN MOIETY OF HUMAN ALPHA-1-ACID GLYCOPROTEIN IN LATE-TERM PREGNANCY

被引：21

作者：

BIOU, D ^{[1
]}

BAUVY, C ^{[1
]}

GUYEN, HN ^{[1
]}

CODOGNO, P ^{[1
]}

DURAND, G ^{[1
]}

AUBERY, M ^{[1
]}

机构：

[1] UER SCI BIOMED ST PERES, PARIS, FRANCE

来源：

CLINICA CHIMICA ACTA | 1991年 / 204卷 / 1-3期

关键词：

ALPHA-1-ACID GLYCOPROTEIN; GLYCAN; PREGNANCY;

D O I：

10.1016/0009-8981(91)90211-T

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

The carbohydrate moiety of purified alpha-1-acid glycoprotein (AGP) from healthy male adults (AGP(n)) and late-term pregnant women (AGP(p)) was analysed. Polyacrylamide gel electrophoresis with sodium dodecyl sulfate before and after N-glycanase treatment showed that AGP(p) had a slightly higher molecular mass due to an enriched carbohydrate moiety. BIO-GEL P-4 and Concanavalin A (Con A)-Sepharose chromatography of the oligosaccharides released by hydrazinolysis and fractionated by high-voltage electrophoresis indicated a progression towards Con A-unbound oligosaccharides and towards larger glycans in pregnancy. Carbohydrate analysis of purified AGP(p) and AGP(n) and of the most increased oligosaccharide fraction (F4A) evidenced a decrease in the fucosyl molar ratio and a slight increase in the galactosyl, N-acetyl-glucosaminyl and N-acetyl neuraminyl ratios. These results suggest that AGP contains more highly branched oligosaccharides and/or additional N-acetyllactosamine-type oligosaccharides in pregnancy.

引用

页码：1 / 12

页数：12

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