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MAMMALIAN CILIARY-BODY EPITHELIAL-CELLS IN CULTURE PRODUCE TRANSFORMING GROWTH-FACTOR-BETA

被引：62
作者：
HELBIG, H
KITTREDGE, KL
COCAPRADOS, M
DAVIS, J
PALESTINE, AG
NUSSENBLATT, RB
机构：
[1] YALE UNIV,SCH MED,DEPT OPHTHALMOL & VISUAL SCI,NEW HAVEN,CT 06510
[2] NEI,IMMUNOL LAB,BETHESDA,MD 20892
来源：
GRAEFES ARCHIVE FOR CLINICAL AND EXPERIMENTAL OPHTHALMOLOGY | 1991年 / 229卷 / 01期
关键词：
D O I：
10.1007/BF00172268
中图分类号：
R77 [眼科学];
学科分类号：
100212 ;
摘要：
Transforming growth factor-beta (TGF-beta) has recently been found in the aqueous humor. The present study was initiated to test whether the ciliary-body epithelium, the site of aqueous humor formation, is capable of producing TGF-beta. Human, rat and bovine ciliary epithelial cells were grown in tissue culture and their supernatants were tested for TGF-beta using a bioassay. After acid activation of the samples, TGF-beta activity was found in the supernatants of all three mammalian ciliary epithelial cells. Most of this activity could be blocked by a neutralizing antibody against TGF-beta type 2. Native supernatants did not contain detectable TGf-beta activity. Thus, the ciliary epithelium is capable of producing the inactive form of TGf-beta (mainly type 2) and may be a source of TGF-beta in the aqueous humor.
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页码：84 / 87
页数：4
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