RAPID EXTRACTION OF HIGH-PURITY CHROMOSOMAL DNA FROM SERRATIA-MARCESCENS

被引：10

作者：

ALONSO, R ^{[1
]}

NICHOLSON, PS ^{[1
]}

PITT, TL ^{[1
]}

机构：

[1] DIV HOSP INFECT,CENT PUBL HLTH LAB,LONDON NW9 5HT,ENGLAND

来源：

LETTERS IN APPLIED MICROBIOLOGY | 1993年 / 16卷 / 02期

关键词：

D O I：

10.1111/j.1472-765X.1993.tb00348.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Rapid non-specific degradation of Serratia marcescens DNA extracted with guanidium thiocyanate, occurred within 10 min of incubation with restriction endonuclease enzymes. The described modified method based on chemical and enzymatic deproteinization produced preparations of Ser. marcescens DNA of high yield and quality which did not autodegrade when incubated with restriction endonucleases.

引用

页码：77 / 79

页数：3