INSULIN-LIKE GROWTH-FACTOR-I INCREASES MYELINATION AND INHIBITS DEMYELINATION IN CULTURED ORGANOTYPIC NERVE-TISSUE

被引：33

作者：

ROTH, GA ^{[1
]}

SPADA, V ^{[1
]}

HAMILL, K ^{[1
]}

BORNSTEIN, MB ^{[1
]}

机构：

[1] YESHIVA UNIV ALBERT EINSTEIN COLL MED,ROSE F KENNEDY CTR RES MENTAL RETARDAT & HUMAN DEV,BRONX,NY 10461

来源：

DEVELOPMENTAL BRAIN RESEARCH | 1995年 / 88卷 / 01期

关键词：

INSULIN-LIKE GROWTH FACTOR; INSULIN; MYELINATION; DEMYELINATION; REMYELINATION; NERVE TISSUE; EXPERIMENTAL ALLERGIC ENCEPHALOMYELITIS;

D O I：

10.1016/0165-3806(95)00088-U

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The implication of insulin-like growth factor I(IGF-I) in the myelination and the repair of myelin that occur after a demyelinating process was evaluated in organotypic cultures of embryonic nerve tissue. The amount of myelin of mouse spinal cord explants exposed to media supplemented with IGF-I beginning on the first day of explantation was recorded by light-microscopic examination and quantitation of the 2',3'-cyclic nucleotide 3'-phosphohydrolase (CNPase) activity. After 9 days in vitro (DIV), the cultures treated with medium supplemented with 0.1-1 mu g/ml IGF-I showed a greater amount of myelin and an increase over the controls in CNPase activity between 50 and 80% at 16 DIV and 100% at 21 DIV. Total demyelination with a concomitant reduction of about 80% in the CNPase activity resulted when anti-white matter antiserum and complement were added to the nutrient medium of fully myelinated cultures. This effect was partially reverted when IGF-I was included in the demyelinating medium. The higher inhibition, about 50%, was obtained with concentrations of IGF-I between 0.1 and 0.5 mu g/ml. To study the effect of IGF-I on remyelination, well-myelinated cultures were completely demyelinated, maintained in that state for 2 or 15 DIV and after that allowed to remyelinate for 14 days. Cultures exposed to medium supplemented with 0.01-0.1 mu g/ml IGF-I showed a degree of remyelination similar to that of the normal nutrient medium-fed cultures. Higher concentration of IGF-I seems to have a negative effect, since the amount myelin did not increases and remained similar to that of the demyelinated groups during all the times tested. The described effect of IGF-I. on the nerve tissue is consistent with a proposed role in the regulation of oligodendroglial and neuronal development.

引用

页码：102 / 108

页数：7

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