DIFFERENTIAL EXPRESSION OF 2 TESTIS-SPECIFIC TRANSCRIPTS OF THE MOUSE PDHA-2 GENE DURING SPERMATOGENESIS

被引：17

作者：

FITZGERALD, J ^{[1
]}

DAHL, HHM ^{[1
]}

IANNELLO, RC ^{[1
]}

机构：

[1] ROYAL CHILDRENS HOSP, MURDOCH INST RES BIRTH DEFECTS, MELBOURNE, VIC 3052, AUSTRALIA

来源：

DNA AND CELL BIOLOGY | 1994年 / 13卷 / 05期

关键词：

D O I：

10.1089/dna.1994.13.531

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Analysis of the expression of the testis-specific isoform of the mouse pyruvate dehydrogenase E1 alpha subunit gene (Pdha-2) during various stages of spermatogenesis has shown that a 2.0-kb Pdha-2 mRNA is initially transcribed in meiotic prophase. The initial appearance of Pdha-2 mRNA precedes that of Pgk-2 and corresponds to the appearance of Ldh-3 mRNA. A second Pdha-2 1.7-kb transcript is present in post-meiotic round spermatids. Polysomal analysis of purified spermatogenic cell populations demonstrates that the 2.0-kb mRNA species is translated in diploid, pachytene spermatocytes and the 1.7-kb mRNA species is translated in round spermatids, although a large proportion is present on the nonpolysomal fraction and may be stored for use in later stages of spermiogenesis.

引用

页码：531 / 537

页数：7

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