ELECTROTRANSFER OF MICROSOMAL CYTOCHROME-P450 AFTER ISOELECTRIC-FOCUSING (IEF BLOTS) - RESOLUTION OF HUMAN 2A-ISOENZYMES AND 3A-ISOZYMES

被引：7

作者：

BONFILS, C ^{[1
]}

DAUJAT, M ^{[1
]}

CHEVRON, MP ^{[1
]}

DALETBELUCHE, I ^{[1
]}

机构：

[1] CNRS,INSERM,CTR RECH BIOCHIM MACROMOLEC,F-34033 MONTPELLIER,FRANCE

来源：

ANALYTICAL BIOCHEMISTRY | 1994年 / 218卷 / 01期

关键词：

D O I：

10.1006/abio.1994.1143

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In this article a procedure is described which allows us to achieve electroblots of membrane-associated proteins after isoelectric focusing. Proteins are resolved in vertical acrylamide slab gels containing ampholines, urea, and nonionic detergents (dodecyl maltoside and Tergitol NP10). After migration the focusing gel is reinforced by a carrier gel (10% acrylamide) polymerized on only one face of the slab. Electroelution of the proteins toward a hydrophobic membrane (Immobilon P) is obtained by adding denaturing agents to the carrier gel (sodium dodecyl sulfate and mercaptoethanol) and by increasing the methanol concentration (60%) in the transfer buffer. This method is applied to analyzing P450 2A and 3A cytochromes from human liver microsomes. (C) 1994 Academic Press, Inc.

引用

页码：80 / 86

页数：7

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