IDENTIFICATION AND ANALYSIS OF THE START SITE OF RIBOSOMAL-RNA TRANSCRIPTION OF ENTAMOEBA-HISTOLYTICA

被引：12

作者：

MICHEL, B

LIZARDI, PM

ALAGON, A

ZURITA, M

机构：

[1] UNIV NACL AUTONOMA MEXICO,DEPT GENET & FISIOL MOLEC,CUERNAVACA 62271,MORELOS,MEXICO

[2] UNIV NACL AUTONOMA MEXICO,INST BIOTECNOL,CUERNAVACA 62271,MORELOS,MEXICO

[3] UNIV NACL AUTONOMA MEXICO,INST BIOTECNOL,DEPT RECONOCIMIENTO MOLEC & BIOESTRUCT,CUERNAVACA 62271,MORELOS,MEXICO

来源：

MOLECULAR AND BIOCHEMICAL PARASITOLOGY | 1995年 / 73卷 / 1-2期

关键词：

ENTAMOEBA HISTOLYTICA; RIBOSOMAL-RNA TRANSCRIPTION START SITE; TANDEM REPEAT ELEMENT;

D O I：

10.1016/0166-6851(95)00084-E

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In this article we report the identification of the start site of ribosomal RNA transcription unit of the enteric parasite E. histolytica. We cloned the upstream region of the ribosomal RNA and we defined the 5' boundary of the transcription unit with nuclear run-on assays. We report that ribosomal transcription starts 2447 bp upstream the SSU ribosomal gene, at an adenosine residue. This data was supported both by S1 mapping and by primer extension analysis; that the mapped site was indeed the transcription start point was demonstrated by RNAse protection of the in vitro capped RNA. Our sequence data around the transcription start point shows two different tandem repeat clusters immediately downstream from the transcription start point.

引用

页码：19 / 30

页数：12

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