METHIONINE-ALPHA-NAPHTHYL ESTER, A USEFUL CHROMOGENIC SUBSTRATE FOR ESTERPROTEASES OF THE MOUSE SUB-MANDIBULAR GLAND

The two aminoacid esters, N-acetyl-l-methionine α-naphthyl ester and N-acetyl-l-alanine α-naphthyl ester have been found to be suitable chromogenic substrates for the demonstration of mouse submandibular esterproteases. Using these substrates, a complex banding pattern of esterproteases was demonstrated by disc electrophoresis of mouse submandibular gland. Of these, protease A, epidermal growth factor binding protein, and the γ-subunits of 7 S nerve growth factor could be identified. © 1979 Academic Press, Inc.