THE DEVELOPMENT OF MONOCLONAL-ANTIBODIES AGAINST SALMON (ONCORHYNCHUS-KISUTCH AND ONCORHYNCUS-KETA) PITUITARY-HORMONES AND THEIR IMMUNOHISTOCHEMICAL IDENTIFICATION

被引:18
作者
FARBRIDGE, KJ
MCDONALDJONES, G
MCLEAN, CL
LOWRY, PJ
ETCHES, RJ
LEATHERLAND, JF
机构
[1] UNIV GUELPH,DEPT ANIM & POULTRY SCI,GUELPH N1G 2W1,ONTARIO,CANADA
[2] ST BARTHOLOMEWS HOSP,PROT HORMONE UNIT,LONDON EC1A 7BE,ENGLAND
[3] UNIV READING,DEPT PHYSIOL & BIOCHEM,READING RG6 2AJ,BERKS,ENGLAND
关键词
D O I
10.1016/0016-6480(90)90066-U
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Monoclonal antibodies (MCAs) directed against several salmon pituitary hormones were generated by the fusion of myeloma cells with spleen cells from mice that had been immunized with either chum salmon (Oncorhynchus keta) growth hormone (GH) or prolactin (PRL), or one of two purified protein preparations from coho salmon (O. kisutch) pituitaries. Hybridoma were cloned by limiting dilution and screened for MCA production using immunohistochemical procedures. MCAs were generated that bound specifically to GH, PRL, or gonadotropic cells. MCAs were generated that bound to either fine granular material or large globular inclusions in the cytoplasm of the "classical" strongly PAS-positive globular gonadotropic cell type found in mature fish. This suggests that these MCAs are directed against gonadotropin II (GTH II). A MCA was also generated that bound both granular and globular material in the globular gonadotrops and granular material in the weakly PAS-positive vesicular gonadotrops in pituitaries from mature fish and to a cell type in immature rainbow trout pituitaries which is tentatively identified as the gonadotropin I (GTH I) cell type. This MCA did not bind to thyrotrops in immature rainbow trout pituitaries. © 1990.
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页码:361 / 374
页数:14
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