PROTEIN RECOVERY FROM REVERSED MICELLAR SOLUTIONS THROUGH CONTACT WITH A PRESSURIZED GAS-PHASE

被引：54

作者：

PHILLIPS, JB ^{[1
]}

NGUYEN, H ^{[1
]}

JOHN, VT ^{[1
]}

机构：

[1] TULANE UNIV,DEPT CHEM ENGN,NEW ORLEANS,LA 70118

来源：

BIOTECHNOLOGY PROGRESS | 1991年 / 7卷 / 01期

关键词：

D O I：

10.1021/bp00007a007

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We describe a new process for the recovery of encapsulated protein from reversed micellar solution in concentrated form. The method involves desolubilization of the protein by decreasing solvent density through gas dissolution. Under appropriate thermodynamic conditions, the micellar water pool can be converted to clathrate hydrates. Protein recovery is facilitated by clathrate hydrate formation, which causes the desolubilized protein to exist in a solid phase, distinct from the micellar supernatant. The process is carried out without any ionic strength or pH modification.

引用

页码：43 / 48

页数：6

共 22 条

[1] Battistel E., Luiai P.L., Rialdi G., Thermodynamic Study of Globular Protein Stability in Microemulsions, J. Phys. Chem., 92, pp. 6680-6685, (1988)
[2] Berecz E., Balla-Achs M., Studies in Inorganic Chemistry‐4: Gas Hydrates, (1983)
[3] Eastoe J., Robinson B.H., Steytler D.C., Influence of Pressure and Temperature on Microemulsion Stability, J. Chem. Sec., Faraday Trans. 1, 86, pp. 511-517, (1990)
[4] Eastoe J., Young W.K., Robinson B.H., Steytler D.C., Scattering Studies of Microemulsions in Low‐Density Alkanes, J. Chem. Soc, Faraday Trans. 1, 86, pp. 2883-2889, (1990)
[5] Goklen K.E., Hatton T.A., Liquid‐Liquid Extraction of Low Molecular‐Weight Proteins by Selective Solubilization in Reversed Micelles, Separation Science and Technology, 22, pp. 831-841, (1987)
[6] Holder G.D., Hand J.B., Multiple‐Phase Equilibria in Hydrates from Methane, Ethane, Propane, and Water Mixtures, AIChE J., 28, pp. 440-447, (1982)
[7] Jain T.K., Varshney M., Maitra A., Structural Studies of Aerosil OT Reverse Micellar Aggregates by FT‐IT Spectroscopy, J. Phys. Chem., 93, pp. 7409-7416, (1989)
[8] John V.T., Papadopoulos K.D., Holder G.D., A Generalized Model for Predicting Equilibrium Conditions for Gas Hydrates, AIChE J., 31, pp. 252-259, (1985)
[9] Johnston K.P., McFann G.J., Lemert R.M., Pressure Tuning of Reverse Micelles for Adjustable Solvation of Hydrophiles in Supercritical Fluids, Supercritical Fluid Science and Technology, pp. 140-154, (1989)
[10] Lang J., Jada A., Malliaris A., Structure and Dynamics of Water‐in‐Oil Droplets Stabilized by Sodium Bis(2‐ethylhexyl) Sulfosuccinate, J. Phys. Chem., 92, pp. 1946-1953, (1988)

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