CONTROL OF GENE-EXPRESSION IN THE TEMPERATE COLIPHAGE-186 .9. B IS THE SOLE PHAGE FUNCTION NEEDED TO ACTIVATE TRANSCRIPTION OF THE PHAGE LATE GENES

被引:12
作者
DIBBENS, JA [1 ]
EGAN, JB [1 ]
机构
[1] UNIV ADELAIDE, DEPT BIOCHEM, ADELAIDE, SA 5000, AUSTRALIA
关键词
D O I
10.1111/j.1365-2958.1992.tb01440.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Using plasmid clones we have determined that the late control function B is the only phage function that is needed to activate a late promoter of coliphage 186, and we predict that it functions as an auxiliary factor to RNA polymerase in the activation of late transcription. We have also shown that a high concentration of B will activate late transcription from a prophage, and we conclude that replicating DNA is not a template requirement for B to function. The original demonstration of a need for the replication gene A in late transcription can be explained by the fact that replication leads to an increase in B gene dosage, with the consequent increase in B concentration leading to the efficient activation of the late promoters.
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页码:2629 / 2642
页数:14
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