CA2+-DEPENDENT PROTEIN-KINASE-C ISOFORMS IN RAT PITUITARY HYPERPLASIA - EFFECT OF IN-VIVO TREATMENT WITH QUINAGOLIDE

Ca2(+)-dependent protein kinase C (PKC) activity, diacylglycerol levels and PKC alpha, beta(I), beta(II), and gamma expression were analyzed in the pituitary of female rats treated with estradiol alone (2 months) or in combination with quinagolide in the second month. Polymerase chain reaction (PCR) and Western blot analysis revealed the presence of PKC alpha, beta(I) and beta(II) isoenzymes in the rat pituitary gland but not of PKC gamma isoenzymes. Increases in pituitary weight and plasma prolactin levels induced by estradiol were associated with an increase in diacylglycerol pituitary content (1.55 +/- 0.06 versus 1.12 +/- 0.17 nmol diacylglycerol/mg protein in controls, P < 0.01). Cotreatment with quinagolide reversed these effects. Changes in PKC activity were accompanied by parallel changes in PKC alpha and beta(I) expressions. Estradiol treatment increased the expression of these isoforms whereas cotreatment with quinagolide antagonized these effects. PKC beta(II) expression was not affected. In conclusion, Ca2+-dependent PKC activity and protein expression are increased in hyperplastic pituitary cells, suggesting the involvement of this class of PKCs in the rat pituitary cell proliferation and/or hormonal secretion. This is further assessed by the fact that the dopamine receptor agonist treatment decreases activity and expression of these PKCs in parallel with the decrease in hormonal secretion and cell proliferation.