PROPERTIES OF NEW ESCHERICHIA-COLI HFR STRAINS CONSTRUCTED BY INTEGRATION OF PSC101-DERIVED CONJUGATIVE PLASMIDS

被引：8

作者：

FRANCOIS, V ^{[1
]}

CONTER, A ^{[1
]}

LOUARN, JM ^{[1
]}

机构：

[1] CNRS,CTR BIOCHIM & GENET CELLULAIRES,118 ROUTE NARBONNE,F-31062 TOULOUSE,FRANCE

来源：

JOURNAL OF BACTERIOLOGY | 1990年 / 172卷 / 03期

关键词：

D O I：

10.1128/jb.172.3.1436-1440.1990

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Conjugative temperature-sensitive plasmids were derived from pSC101. These plasmids are useful in genetic analysis for two reasons: (i) they render possible the construction of new Hfr lines by plasmid integration at predetermined chromosomal loci via Tn10 inverse transposition, and (ii) the Hfr characters are transducible via bacteriophage P1. We also showed that replication from pSC101 origin is deleterious for the plasmid-chromosome fusion.

引用

页码：1436 / 1440

页数：5

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