COMPUTERIZED DETERMINATION OF GROWTH KINETIC CURVES AND DOUBLING TIMES FROM CELLS IN MICROCULTURE

被引：112

作者：

REILE, H

BIRNBOCK, H

BERNHARDT, G

SPRUSS, T

SCHONENBERGER, H

机构：

[1] Institut für Pharmazie, Sonderforschungsbereich 234, Universität Regensburg

来源：

ANALYTICAL BIOCHEMISTRY | 1990年 / 187卷 / 02期

关键词：

D O I：

10.1016/0003-2697(90)90454-H

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In this paper we describe the microcomputer-aided determination of cell proliferation kinetics and doubling times utilizing a crystal violet assay and a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide assay in microtitration plates. The analysis of spectrophotometric data provides the doubling times at any time of incubation. Plots of doubling time versus time of incubation give reproducible information on the exact duration of the logarithmic growth phase. This method is applicable to anchorage-dependent as well as anchorage-independent cells when colorimetric or fluorometric data are accessible. © 1990.

引用

页码：262 / 267

页数：6

共 18 条

[1]

ALLEY MC, 1988, CANCER RES, V48, P589

[2]

CARMICHAEL J, 1987, CANCER RES, V47, P936

[3] RAPID COLORIMETRIC ASSAY FOR CELL-GROWTH AND SURVIVAL - MODIFICATIONS TO THE TETRAZOLIUM DYE PROCEDURE GIVING IMPROVED SENSITIVITY AND RELIABILITY [J].