SELECTIVITY OF NUCLEOSIL-10 NH2 AS AN ADSORBENT IN HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

Net retention volumes per gram of Nucleosil 10 NH2 have been measured for a large number of mono- and disubstituted benzene derivatives and of polycyclic aromatic hydrocarbons, using n-hexane, dichloromethane and a mixture of both as eluents at 25°. The retention data are interpreted in terms of the semi-empirical adsorption model, developed by Snyder for bare adsorbents, using octadecylsilylsilica as a reference adsorbent. The effects of the bound monomers on adsorbent deactivation, solute and eluent localization, change of the charge distribution in the solute molecule and adsorption mode of the solute are evaluated and discussed in terms of donor-acceptor interaction (including hydrogen bonding). © 1979.