IMMUNOCYTOCHEMICAL LOCALIZATION OF ENDOTHELIN IN CULTURED BOVINE ENDOTHELIAL-CELLS

被引：90

作者：

NAKAMURA, S ^{[1
]}

NARUSE, M ^{[1
]}

NARUSE, K ^{[1
]}

DEMURA, H ^{[1
]}

UEMURA, H ^{[1
]}

机构：

[1] TOKYO WOMENS MED COLL,INST CLIN ENDOCRINOL,DEPT MED,TOKYO 162,JAPAN

来源：

HISTOCHEMISTRY | 1990年 / 94卷 / 05期

关键词：

D O I：

10.1007/BF00272609

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

To investigate the intracellular localization of endothelin in cultured endothelial cells, an immunocytochemical study was carried out by the post-embedding protein A-gold technique with endothelin-specific antiserum. Gold particles were seen on the rough endoplasmic reticulum, the Golgi cisternae, the Golgi vesicles, small vesicles beneath the cell membrane, and the lysosomes. By contrast, no secretory granules were observed. These results suggest that endothelin is secreted by a constitutive pathway and that the lysosome may play an important role in regulating the biological activity of endothelin. © 1990 Springer-Verlag.

引用

页码：475 / 477

页数：3

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