A RAPID AND EFFICIENT NEW ASSAY FOR DETERMINATION OF THE 3 BIOTYPES OF AGROBACTERIUM-TUMEFACIENS

被引：5

作者：

BUSH, AL ^{[1
]}

PUEPPKE, SG ^{[1
]}

机构：

[1] UNIV MISSOURI,DEPT PLANT PATHOL,COLUMBIA,MO 65211

来源：

LETTERS IN APPLIED MICROBIOLOGY | 1991年 / 13卷 / 03期

关键词：

D O I：

10.1111/j.1472-765X.1991.tb00588.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We describe a new method for determining the biotype of Agrobacterium tumefaciens strains, based on differential motility in a medium adjusted to different pH levels. Motility of biotype I increases with increasing pH. That of biotype II slightly decreases, but the cells remain motile; the motility of biotype III decreases and the cells become non-motile. The assay is able to distinguish between strains of all three biotypes within 2 d, and can be applied to relatively large numbers of isolates. Thus it is more rapid and efficient than the frequently used biochemical biotype tests.

引用

页码：126 / 129

页数：4

共 9 条

[1] BEHAVIORAL MUTANTS OF RHIZOBIUM-MELILOTI [J].