GENETIC-MAPPING OF 1,3-BETA-GLUCANASE-ENCODING GENES IN SACCHAROMYCES-CEREVISIAE

被引：13

作者：

CORREA, J ^{[1
]}

DEALDANA, CRV ^{[1
]}

SEGUNDO, PS ^{[1
]}

DELREY, F ^{[1
]}

机构：

[1] UNIV SALAMANCA,CSIC,FAC BIOL,INST MICROBIOL BIOQUIM,E-37008 SALAMANCA,SPAIN

来源：

CURRENT GENETICS | 1992年 / 22卷 / 04期

关键词：

1,3-BETA-GLUCANASE GENES; SACCHAROMYCES-CEREVISIAE; CHROMOSOMAL MAPPING; GENETIC MAPPING;

D O I：

10.1007/BF00317922

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The map position of three 1,3-beta-glucanase-encoding genes in S. cerevisiae has been determined following conventional meiotic and mitotic mapping combined with recombinant DNA techniques. EXG1, EXG2 and SSG1 were localized to chromosomes XII, IV and XV, respectively, by hybridizing the cloned genes to Southern blots of chromosomes separated by pulsed-field gel electrophoresis, in conjunction with the rad52-1-dependent chromosome-loss mapping technique. Meiotic tetrad analyses further localized the EXG1 gene 6.1 centimorgans centromere-proximal to CDC25 on the right arm of chromosome XII. EXG2 was positioned between LYS4 and GCN2 on the right arm of chromosome IV, at distances of 6.2 centimorgans from LYS4 and 4.9 centimorgans from GCN2. Finally, the SSG1 locus mapped on the right arm of chromosome XV, about 8.2 centimorgans to the centromere-proximal side of HIS3.

引用

页码：283 / 288

页数：6

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