BLOCK TO DEVELOPMENT IN CULTURED RAT 1-CELL EMBRYOS IS OVERCOME USING MEDIUM HECM-1

被引：77

作者：

KISHI, J

NODA, Y

NARIMOTO, K

UMAOKA, Y

MORI, T

机构：

[1] Department of Gynaecology and Obstetrics, Faculty of Medicine, Kyoto University, Sakyo-ku, Kyoto 606-01

来源：

HUMAN REPRODUCTION | 1991年 / 6卷 / 10期

关键词：

DEVELOPMENTAL BLOCKAGE; GLUCOSE; OXYGEN; PHOSPHATE; RAT EMBRYO;

D O I：

10.1093/oxfordjournals.humrep.a137286

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

Rat pronuclear embryos were cultured in hamster embryo culture medium-1 (HECM-1) or a modified Krebs-Ringer bicarbonate solution (mKRB). Embryo cultures in HECM-1 were also challenged with low oxygen concentrations. In HECM-1, 57.9% (70/121) of the pronuclear embryos developed into the 4-cell stage after 48 h of culture. The rates of 8-cell, morula and blastocyst formation were 32.2% (39/121), 17.4% (21/121) and 9.9% (12/121), respectively. On the other hand, in mKRB, rat pronuclear embryos showed developmental blockages at the 2-cell and 4-cell stages, and never developed beyond the 4-cell stage. The rate of blastocyst formation under a low oxygen concentration was 20.1% (43/214), showing a significant difference from the value of 5.5% (11/201) obtained under a standard oxygen concentration (P < 0.005). This is the first report of successful culture of rat pronuclear embryos to the blastocyst stage. Furthermore, it is suggested that protection from oxidation stress is a prerequisite for rat embryo development in vitro.

引用

页码：1445 / 1448

页数：4

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