ACTIVATION OF OXIDIZED CYSTEINE PROTEINASES BY THIOREDOXIN-MEDIATED REDUCTION INVITRO

被引：18

作者：

STEPHEN, AG ^{[1
]}

POWLS, R ^{[1
]}

BEYNON, RJ ^{[1
]}

机构：

[1] UNIV LIVERPOOL,DEPT BIOCHEM,PROTEOLYSIS RES GRP,POB 147,LIVERPOOL L69 3BX,ENGLAND

来源：

BIOCHEMICAL JOURNAL | 1993年 / 291卷

关键词：

D O I：

10.1042/bj2910345

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Activity of the cysteine adducts of the cysteine proteinases papain and thaumatopain can be recovered by treatment with thioredoxin, thioredoxin reductase and NADPH. Recovery of proteinase activity did not occur if any of the components of the thioredoxin system were omitted, or if thioredoxin or thioredoxin reductase were heat-inactivated. Such an enzyme-mediated process may be of significance in the recovery of cysteine proteinases inactivated by oxidative attack.

引用

页码：345 / 347

页数：3

共 21 条

[1] L-TRANS-EPOXYSUCCINYL-LEUCYLAMIDO(4-GUANIDINO)BUTANE (E-64) AND ITS ANALOGS AS INHIBITORS OF CYSTEINE PROTEINASES INCLUDING CATHEPSINS B, H AND L [J].

BARRETT, AJ ;

KEMBHAVI, AA ;

BROWN, MA ;

KIRSCHKE, H ;

KNIGHT, CG ;

TAMAI, M ;

HANADA, K .

BIOCHEMICAL JOURNAL, 1982, 201 (01) :189-198

[2] PURIFICATION AND CHARACTERIZATION OF VICILIN PEPTIDOHYDROLASE, MAJOR ENDOPEPTIDASE IN COTYLEDONS OF MUNG-BEAN SEEDLINGS [J].

BAUMGARTNER, B ;

CHRISPEELS, MJ .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1977, 77 (02) :223-233

[3]

BUCHANAN BB, 1986, THIOREDOXIN GLUTARED, P233

[4] PURIFICATION AND CHARACTERIZATION OF THAUMATOPAIN, A CYSTEINE PROTEASE FROM THE ARILS OF THE PLANT THAUMATOCOCCUS-DANIELLII [J].

CUSACK, M ;

STEPHEN, AG ;

POWLS, R ;

BEYNON, RJ .

BIOCHEMICAL JOURNAL, 1991, 274 :231-236

[5]

FERDINAN.W, 1965, J BIOL CHEM, V240, P1150

[6]

GORPINCHENKO T V, 1975, Biokhimiya, V40, P323

[7] PROTEIN DISULPHIDE REDUCTASE FROM PEA SEEDS [J].

HATCH, MD ;

TURNER, JF .

BIOCHEMICAL JOURNAL, 1960, 76 :556-562

[8]

HOLMGREN A, 1985, ANNU REV BIOCHEM, V54, P237, DOI 10.1146/annurev.biochem.54.1.237

[9]

HOLMGREN A, 1989, J BIOL CHEM, V264, P13963

[10]

HOLMGREN A, 1984, POSTTRANSLATIONAL MO, P295

← 1 2 3 →