SULFATE CAN INDUCE DIFFERENTIAL EXPRESSION OF THIOGLUCOSIDE GLUCOHYDROLASES (MYROSINASES)

被引:18
作者
BONES, AM
VISVALINGAM, S
THANGSTAD, OP
机构
[1] UNIGEN-Center for Molecular Biology, Department of Botany, MTFS, University of Trondheim, Trondheim
关键词
GENE EXPRESSION (DIFFERENTIAL); MYROSINASE (THIOGLUCOSIDE GLUCOHYDROLASE); SULFATE; (MYROSINASE INDUCTION); SINAPIS;
D O I
10.1007/BF02411562
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Thioglucoside glucohydrolase (EC 3.2.3.1; myrosinase) hydrolyses glucosinolates and thereby liberates glucose and sulphur and nitrogen compounds. To examine the hypothesis that the myrosinase-glucosinolate system is influenced by environmental factors, the effect of sulphate on the expression of myrosinases was examined. On examining different plant organs at various stages, it was observed that sulphate induces a differential expression of myrosinase polypeptides in plants of Sinapis alba L. (white mustard). Specific myrosinase polypeptides, dependent on sulphate in the growth medium, were detected on immunoblots. Without sulphate a maximum of three polypeptides was detected in buds, two in cotyledons and one in stems and roots. In plants cultured on medium with sulphate up to four polypeptides could be observed in cotyledons, five polypeptides in buds, two in stems and one in roots. Expression of myrosinases was, in general, high in plants cultured on a medium supplemented with sulphate. In flowering S. alba plants, sulphate-starved plants showed a higher expression of myrosinase in cotyledons and stems compared to plants fed with sulphate. Sulphate-fed plants had a high expression in inflorescences and roots. The organ- and time-specific induction of the myrosinase expression is discussed in relation to sulphate metabolism and availability of sulphate under normal conditions of cultivation and in relation to protection of Brassicaceae species. This is the first evidence for a specific induction of individual myrosinase proteins.
引用
收藏
页码:558 / 566
页数:9
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