STEPWISE ELONGATION-FACTOR G-PROMOTED ELONGATION OF POLYPEPTIDES ON RIBOSOME WITHOUT GTP CLEAVAGE

被引：23

作者：

BELITSINA, NV ^{[1
]}

GLUKHOVA, MA ^{[1
]}

SPIRIN, AS ^{[1
]}

机构：

[1] ACAD SCI USSR, PROT RES INST, PUSHCHINO OKA, USSR

来源：

JOURNAL OF MOLECULAR BIOLOGY | 1976年 / 108卷 / 03期

关键词：

D O I：

10.1016/S0022-2836(76)80140-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Poly(U)-bound ribosomes [from Escherichia coli] containing presynthesized polypeptide (polyphenylalanyl-tRNA) become competent for binding of the next phenylalanyl-tRNA and subsequent transpeptidation as a result of attachment of elongation factor G [EF-G] with a non-cleavable GTP analog (guanylyl-methylene diphosphonate) and its successive removal from the ribosomes. Hence, EF-G-promoted translocation and resultant elongation of polypeptide can be induced without GTP cleavage. The elongation cycle described can be repeated.

引用

页码：609 / 613

页数：5

共 13 条

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