ENZYMATIC DETERMINATION OF ESTRADIOL AND ESTRONE IN PLASMA AND URINE

被引:47
作者
NICOLAS, JC
BOUSSIOUX, AM
DESCOMPS, B
CRASTESDEPAULET, A
机构
关键词
D O I
10.1016/0009-8981(79)90389-9
中图分类号
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号
1001 ;
摘要
Plasma and urine estradiol and estrone can be determined using the trans-hydrogenase function of the estradiol dehydrogenase of human placenta: in the conditions described the transhydrogenase activity which is directly related to the estrogen concentration is measured by spectrophotometry. Estrone and estradiol can be determined together or separately if estrone is previously reacted with hydrazine with a limit of sensitivity of 10 picograms in the sample. On urine samples the assay is performed directly without extraction, a simplification which makes the method highly suitable for numerous routine determinations. © 1979.
引用
收藏
页码:1 / 9
页数:9
相关论文
共 11 条
  • [1] ADAMS JA, 1962, J BIOL CHEM, V237, P3069
  • [2] DESCOMPS B, 1969, Bulletin de la Societe de Chimie Biologique, V51, P1591
  • [3] ENZYMATIC TECHNIQUES IN STEROID ASSAY
    HARKONEN, M
    ADLERCREUTZ, H
    GROMAN, EV
    [J]. JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY, 1974, 5 (08) : 717 - 725
  • [4] JARABAK J, 1962, J BIOL CHEM, V237, P345
  • [5] LANGER LJ, 1959, J BIOL CHEM, V234, P2609
  • [6] LANGER LJ, 1958, J BIOL CHEM, V233, P583
  • [7] STUDIES ON STEROIDS .123. PICOGRAM ORDER ENZYME IMMUNOASSAY OF ESTRADIOL
    NUMAZAWA, M
    HARYU, A
    KUROSAKA, K
    NAMBARA, T
    [J]. FEBS LETTERS, 1977, 79 (02) : 396 - 398
  • [8] ACTIVATION OF HYDROGEN TRANSFER BETWEEN PYRIDINE NUCLEOTIDES BY STEROID HORMONES
    TALALAY, P
    WILLIAMSASHMAN, HG
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1958, 44 (01) : 15 - 26
  • [9] VANVEEMEN BK, 1972, FEBS LETT, V24, P77
  • [10] VANVEEMEN BK, 1975, IMMUNOCHEMISTRY, V12, P667