INACTIVATION OF THE PLASMINOGEN ACTIVATOR FROM HELA-CELLS BY PEPTIDES OF ARGININE CHLOROMETHYL KETONE

被引：27

作者：

COLEMAN, P

KETTNER, C

SHAW, E

机构：

[1] Biology Department, Brookhaven National Laboratory, Upton

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1979年 / 569卷 / 01期

关键词：

Arginine chloromethyl ketone; Fibrinolysis; Phorbol ester; Plasminogen activator; Urokinase;

D O I：

10.1016/0005-2744(79)90079-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The binding specificities of human urinary urokinase (EC 3.4.99.26) and HeLa cell plasminogen activator were studied using peptidyl chloromethyl ketone inhibitors. A 125I-labeled fibrin assay has been developed to yield kinetic information. Reagents of the sequence X-Gly-ArgCH2Cl were the most effective. The susceptibility of the HeLa cell plasminogen activator differed from that of urokinase in several respects indicating the utility of this type of inhibitor in distinguishing between proteases of this specificity. © 1979.

引用

页码：41 / 51

页数：11

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