GAS CHROMATOGRAPHY OF SOME URINARY ACID METABOLITES RELATED TO PHENYLKETONURIA

A rapid accurate method for the determination of o-hydroxyphenylacetic acid, β-phenyllactic acid, and phenylpyruvic acid has been developed for the analysis of phenylketonuric urine. A semiquantitative measure of the amount of p-hydroxyphenylpyruvic acid is obtained simultaneously. The method uses ethyl acetate extraction of acidified urine, trimethylsilylation of the residue from ethyl acetate evaporation, and gas chromatography of the trimethylsilyl derivatives on a SE-30 column. Normal alkanes are used as internal standards. © 1969.