CHLOROPLAST DNA RESTRICTION-FRAGMENT-LENGTH-POLYMORPHISM IN SEQUOIA-SEMPERVIRENS D DON ENDL, PSEUDOTSUGA-MENZIESII (MIRB) FRANCO, CALOCEDRUS-DECURRENS (TORR), AND PINUS-TAEDA L

被引:19
作者
ALI, IF [1 ]
NEALE, DB [1 ]
MARSHALL, KA [1 ]
机构
[1] US FOREST SERV, PACIFIC SW FOREST & RANGE EXPT STN, INST FOREST GENET, BOX 245, BERKELEY, CA 94701 USA
关键词
CPDNA RFLPS; SEQUOIA-SEMPERVIRENS D DON ENDL; PSEUDOTSUGA-MENZIESII (MIRB) FRANCO; CALOCEDRUS-DECURRENS (TORR) FLORIN; PINUS-TAEDA L;
D O I
10.1007/BF00226116
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
The extent and type of chloroplast DNA restriction fragment length polymorphism was determined among individual tree samples of coast redwood, Douglas fir, incense-cedar, and loblolly pine. A total of 107 trees was surveyed for three restriction enzymes (BamHI, EcoRI, HindIII) and six chloroplast DNA probes from petunia (P3, P4, P6, P8, P10, S8). The probes comprise 64% of the petunia chloroplast genome. Polymorphisms were detected in all species but loblolly pine. Coast redwood and incense-cedar had a small number of rare variants, whereas Douglas fir had one highly polymorphic region of insertions/deletions in sequences revealed by the P6 probe from petunia. The mutation hotspot is currently being studied by DNA sequence analysis.
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页码:83 / 89
页数:7
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