A SIMPLE METHOD OF GENERATING AXENIC DERIVATIVES OF DICTYOSTELIUM STRAINS

被引：12

作者：

MORRISON, A

HARWOOD, A

机构：

[1] The Imperial Cancer Research Fund, Laboratory, South Mimms, Herts EN6 3LD, Clare Hall

来源：

EXPERIMENTAL CELL RESEARCH | 1992年 / 199卷 / 02期

关键词：

D O I：

10.1016/0014-4827(92)90449-I

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

We describe a generally applicable method of adapting Dictyostelium from growth on a bacterial food source to axenic growth. Cells are initially selected by growth on a plastic substratum but subsequently acquire the ability to grow in suspension culture. These strains can be transformed efficiently by DNA-mediated gene transfer. © 1992.

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页码：383 / 386

页数：4

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