AGE AND SEX INFLUENCE EXPRESSION OF PLASMID DNA DIRECTLY INJECTED INTO MOUSE SKELETAL-MUSCLE

被引：64

作者：

WELLS, DJ

GOLDSPINK, G

机构：

[1] Unit of Veterinary Molecular and Cellular Biology, Department of Veterinary Basic Sciences, The Royal Veterinary College, London

来源：

FEBS LETTERS | 1992年 / 306卷 / 2-3期

基金：

英国惠康基金;

关键词：

GENE THERAPY; DNA INJECTION; PLASMID DNA; SKELETAL MUSCLE; SEX; AGE;

D O I：

10.1016/0014-5793(92)81000-C

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Direct injection of plasmid DNA into the skeletal muscle has been proposed as a means of effecting somatic gene therapy. We examined the influence of age and sex on the level of expression of an SV40-CAT construct injected into mouse muscle. Age markedly affected expression, with peak values in the 4-6 week age class which were significantly higher than in animals older than 10 weeks. Sex also altered expression, with higher levels of CAT activity seen in males compared to females. We conclude that the rate of growth is important in determining levels of expression of directly injected DNA.

引用

页码：203 / 205

页数：3

共 12 条

[1]

ACSADI G, 1991, NEW BIOL, V3, P71

[2] HUMAN DYSTROPHIN EXPRESSION IN MDX MICE AFTER INTRAMUSCULAR INJECTION OF DNA CONSTRUCTS [J].

ACSADI, G ;

DICKSON, G ;

LOVE, DR ;

JANI, A ;

WALSH, FS ;

GURUSINGHE, A ;

WOLFF, JA ;

DAVIES, KE .

NATURE, 1991, 352 (6338) :815-818

[3]

ACSADI G, 1990, 5TH INT C INB ERR ME, P73

[4]

CUNLIFFEBEAMER TL, 1987, UFAW HDB CARE MANAGE, P275

[5]

FLECKNELL PA, 1987, LABORATORY ANIMAL AN

[6] STRONG EXPRESSION OF FOREIGN GENES FOLLOWING DIRECT INJECTION INTO FISH MUSCLE [J].

HANSEN, E ;

FERNANDES, K ;

GOLDSPINK, G ;

BUTTERWORTH, P ;

UMEDA, PK ;

CHANG, KC .

FEBS LETTERS, 1991, 290 (1-2) :73-76

[7] DIRECT GENE-TRANSFER INTO NONHUMAN PRIMATE MYOFIBERS INVIVO [J].

JIAO, SS ;

WILLIAMS, P ;

BERG, RK ;

HODGEMAN, BA ;

LIU, LJ ;

REPETTO, G ;

WOLFF, JA .

HUMAN GENE THERAPY, 1992, 3 (01) :21-33

[8] HORMONAL MODULATION OF A GENE INJECTED INTO RAT-HEART INVIVO [J].

KITSIS, RN ;

BUTTRICK, PM ;

MCNALLY, EM ;

KAPLAN, ML ;

LEINWAND, LA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (10) :4138-4142

[9] EXPRESSION OF RECOMBINANT GENES IN MYOCARDIUM INVIVO AFTER DIRECT INJECTION OF DNA [J].

LIN, H ;

PARMACEK, MS ;

MORLE, G ;

BOLLING, S ;

LEIDEN, JM .

CIRCULATION, 1990, 82 (06) :2217-2221

[10]

Sambrook J., 1989, MOL CLONING LAB MANU

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