PENICILLIN ACYLASE EXTRACTION BY OSMOTIC SHOCK

被引：24

作者：

RODRIGUEZ, M ^{[1
]}

GUERECA, L ^{[1
]}

VALLE, F ^{[1
]}

QUINTERO, R ^{[1
]}

LOPEZMUNGUIA, A ^{[1
]}

机构：

[1] UNIV NACL AUTONOMA MEXICO, INST BIOTECNOL, APARTADO POSTAL 510-3, CUERNAVACA 062271, MORELOS, MEXICO

来源：

PROCESS BIOCHEMISTRY | 1992年 / 27卷 / 04期

关键词：

D O I：

10.1016/0032-9592(92)80021-T

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Penicillin acylase was extracted from E. Coli by osmotic shock. The process was optimized by factorial design, scaled up and integrated to a purification process in order to compare it with purification processes reported in the literature. A specific parameter PPEF (Purification Processes Evaluation Factor) was defined for this purpose.

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收藏

页码：217 / 223

页数：7

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