CELL-PROLIFERATION, DNA-REPAIR, AND P53 FUNCTION ARE NOT REQUIRED FOR PROGRAMMED DEATH OF PROSTATIC GLANDULAR CELLS INDUCED BY ANDROGEN ABLATION

被引:207
作者
BERGES, RR
FURUYA, Y
REMINGTON, L
ENGLISH, HF
JACKS, T
ISAACS, JT
机构
[1] JOHNS HOPKINS UNIV,SCH MED,DEPT UROL,BALTIMORE,MD 21205
[2] MIT,CTR CANC RES,CAMBRIDGE,MA 02139
[3] PENN STATE UNIV,MILTON S HERSHEY MED CTR,DIV ENDOCRINOL & UROL,HERSHEY,PA 17033
关键词
APOPTOSIS; S-PHASE PROGRESSION; DNA DAMAGE;
D O I
10.1073/pnas.90.19.8910
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Androgen ablation induces programmed death of androgen-dependent prostatic glandular cells, resulting in fragmentation of their genomic DNA and the cells themselves into apoptotic bodies. Twenty percent of prostatic glandular cells undergo programmed death per day between day 2 and 5 after castration. During this same period, <1% of prostatic glandular cells enter the S phase of the cell cycle, documenting that >95% of these die in G0. During the programmed death of these G0 glandular cells, a futile DNA repair process is induced secondary to the DNA fragmentation. This futile DNA repair is not required, however, since inhibition of this process by >90% with an appropriately timed hydroxy-urea dosing regimen had no effect upon the extent of the programmed death of these cells after castration. Likewise, p53 gene expression is not required since the same degree of cell death occurred in prostates and seminal vesicles after castration of wild-type and p53-deficient mice.
引用
收藏
页码:8910 / 8914
页数:5
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