SEQUENCE AND CHARACTERIZATION OF AN INSERTION-SEQUENCE, IS711, FROM BRUCELLA-OVIS

被引:85
作者
HALLING, SM
TATUM, FM
BRICKER, BJ
机构
[1] National Animal Disease Center, Agricultural Research Service, United States Department of Agriculture, Ames
关键词
SEQUENCE DIVERGENCE; INVERTED REPEATS; TARGET SITE; TRANSPOSASE; FRAMESHIFTING; BRUCELLOSIS;
D O I
10.1016/0378-1119(93)90236-V
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
The nucleotide (nt) sequence of a previously discovered insertion in Brucella ovis was determined and found to have the hallmarks of an insertion sequence (IS). The element, designated IS711, of 842 bp, is similar in G + C content to that of the Brucella genome and is bounded by 20-bp imperfect inverted repeats (IR). The element appears to duplicate the nt TA of a consensus target site, YTAR (R, purines; Y, pyrimidines). When the complete nt sequence of four elements and 300 bp of the 3' ends of five other elements were compared to IS711 and to each other, minor nt sequence variations were found amongst most of them. Similar to several other transposable elements, IS711 has overlapping ORFs rather than one long ORF extending the length of the element. Even though only ten B. ovis IS711 elements were characterized, in three cases we found these elements flanked by either identical or similar nt sequences. This suggests that some target sites are hot spots for insertion and that some of the elements may be duplicated by mechanisms other than transposition. No DNA or protein database entries had an obvious resemblance to either IS711 or its deduced gene products.
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页码:123 / 127
页数:5
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