PRELIMINARY-X-RAY DIFFRACTION ANALYSIS OF CRYSTALS OF TOMATO ASPERMY VIRUS (TAV)

被引：2

作者：

CANADY, MA ^{[1
]}

LEJA, CA ^{[1
]}

DAY, J ^{[1
]}

MCPHERSON, A ^{[1
]}

机构：

[1] UNIV CALIF RIVERSIDE,DEPT BIOCHEM,RIVERSIDE,CA 92521

来源：

PROTEINS-STRUCTURE FUNCTION AND GENETICS | 1995年 / 21卷 / 03期

关键词：

T = 3 PLANT VIRUS; CUCUMOVIRUS; PROTEIN CRYSTALLIZATION; X-RAY DIFFRACTION;

D O I：

10.1002/prot.340210310

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Tomato aspermy virus (TAV) is a member of the T = 3 cucumovirus group, and the chrysanthemum strain (C-TAV) has been crystallized in a form suitable for X-ray structural analysis. The crystals, which grow in 14-17% ethanol at pH 8.5, are of orthorhombic space group I222 with unit cell dimensions of a = 295.1 Angstrom, b = 320.5 Angstrom, and c = 383.6 Angstrom. There are two T = 3 virus particles in the unit cell, which means that they must be centered at 0,0,0 and 1/2, 1/2, 1/2 with icosahedral 222 symmetry elements coincident with crystallographic symmetry operators. The asymmetric unit of the crystals, therefore, contains one quarter of a virus particle, or 45 capsid subunits. Native diffraction data to 4 Angstrom resolution have been collected using synchrotron radiation, though data appear to be present beyond that resolution. (C) 1995 Wiley-Liss, Inc.

引用

页码：265 / 267

页数：3

共 14 条

[1] ASPERMY - A NEW VIRUS DISEASE OF THE TOMATO [J].