PURIFICATION OF CHICKEN PANCREAS NUCLEASE BY SUBSTRATE ELUTION FROM PHOSPHORYLATED CELLULOSE

被引：13

作者：

ELEY, J

机构：

[1] Department of Biology, Rensselaer Polytechnic Institute, Troy

来源：

BIOCHEMISTRY | 1969年 / 8卷 / 04期

关键词：

D O I：

10.1021/bi00832a028

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A nuclease from chicken pancreas adsorbed on phosphorylated cellulose can be specifically eluted by the substrate, ribonucleic acid, and is purified 60-70-fold by this single step. Ribonucleic acid which has been thoroughly hydrolyzed with KOH or with chicken pancreas nuclease will not elute the enzyme. Deoxyribonucleic acid, a poor substrate of the enzyme, and ribonucleic acid which has been thoroughly digested with bovine pancreatic ribonuclease will also elute the enzyme, but the elution is much less sharp than that obtained with ribonucleic acid. The ribonucleic acid can be removed from the phosphorylated cellulose eluent by readsorption of the enzyme onto fresh phosphorylated cellulose and subsequent salt elution. The negatively charged ribonucleic acid is not retained by the cation exchanger. © 1969, American Chemical Society. All rights reserved.

引用

页码：1502 / &

共 10 条

[1]

ARSENIS C, 1966, J BIOL CHEM, V241, P330

[2]

ARSENIS C, 1964, J BIOL CHEM, V239, P3093

[3]

ELEY J, 1966, J BIOL CHEM, V241, P3063

[4]

ELEY J, 1966, J BIOL CHEM, V241, P3070

[5] INFECTIVITY OF RIBONUCLEIC ACID FROM TOBACCO MOSAIC VIRUS [J].