INSITU EXTRACTIVE FERMENTATION OF ACETONE AND BUTANOL

被引:92
作者
ROFFLER, SR
BLANCH, HW
WILKE, CR
机构
[1] Univ of California, Berkeley, CA,, USA, Univ of California, Berkeley, CA, USA
关键词
ACETONE - Biosynthesis - BACTERIOLOGY - Industrial Applications - BIOREACTORS - Fermenters - BIOTECHNOLOGY - SEPARATION - In Situ;
D O I
10.1002/bit.260310207
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The productivity of the acetone-butanol fermentation was increased by continuously removing acetone and butanol from the fermentation broth during fed-batch culture. Whole broth containing viable cells of Clostridium acetobutylicum was cycled to a Karr reciprocating plate extraction column in which acetone and butanol were extracted into oleyl alcohol flowing counter-currently through the column. By continuously removing these toxic metabolites from the broth, end product inhibition was reduced, and a concentrated feed solution containing 300 g/L glucose was fermented at an overall butanol productivity of 1. 0 g/L h, 70% higher than the productivity of normal batch fermentation. The continuous extraction process provides flexible operation and lends itself to process scale-up.
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页码:135 / 143
页数:9
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