FURTHER ANALYSIS OF ATP-MEDIATED ACTIVATION OF K+ CHANNELS IN RENAL EPITHELOID MADIN DARBY CANINE KIDNEY (MDCK) CELLS

被引:11
作者
FRIEDRICH, F
WEISS, H
PAULMICHL, M
WOLL, E
WALDEGGER, S
LANG, F
机构
[1] UNIV INNSBRUCK,INST PHYSIOL,FRITZ PREGL STR 3,A-6020 INNSBRUCK,AUSTRIA
[2] CLIN ANAESTHESIA & GEN INTENS CARE MED,A-6020 INNSBRUCK,AUSTRIA
来源
PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY | 1991年 / 418卷 / 06期
关键词
MDCK CELLS; K+ CHANNELS; ATP ACTIVATION; PHORBOL ESTER; PERTUSSIS TOXIN;
D O I
10.1007/BF00370570
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
ATP activates K+ channels by increasing intracellular calcium activity in Madin Darby canine kidney (MDCK) cells. The present study has been performed to test for the involvement of G-proteins and of protein kinase C in the intracellular transmission of these effects. To this end, the effect of ATP on intracellular calcium and K+ channel activity has been studied in cells pretreated with the phorbol ester 12-O-tetradecanoylphorbol 13-acetate (TPA) and/or pertussis toxin. The ATP-induced increase of intracellular calcium is not significantly affected by pretreatment with pertussis toxin, is significantly blunted by pretreatment with TPA and is abolished by pretreatment with both pertussis toxin and the phorbol ester. The ATP activation of K+ channels is similarly blunted by pretreatment with TPA, but is not abolished by pretreatment with both the phorbol ester and pertussis toxin. Furthermore, the ATP-induced hyperpolarization is not abolished in cells pretreated with both pertussis toxin and TPA. In those cells, ATP may activate K+ channels by calcium-independent mechanisms or lead to localized increases of intracellular calcium sufficient to activate the K+ channels but escaping detection with fura-2 fluorescence.
引用
收藏
页码:551 / 555
页数:5
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