SALT-DEPENDENT PROTEIN-PHOSPHORYLATION IN THE CYANOBACTERIUM SYNECHOCYSTIS PCC-6803

被引：11

作者：

HAGEMANN, M ^{[1
]}

GOLLDACK, D ^{[1
]}

BIGGINS, J ^{[1
]}

ERDMANN, N ^{[1
]}

机构：

[1] BROWN UNIV,BIOCHEM SECT,PROVIDENCE,RI 02912

来源：

FEMS MICROBIOLOGY LETTERS | 1993年 / 113卷 / 02期

基金：

美国国家科学基金会;

关键词：

SALT ADAPTATION; CYANOBACTERIA; PROTEIN PHOSPHORYLATION; SYNECHOCYSTIS PCC-6803;

D O I：

10.1111/j.1574-6968.1993.tb06515.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Hyper- and hypo-osmotic salt shocks in the presence of [P-32]PO43- led to the appearance and disappearance of several P-32-labelled proteins in vivo within a few minutes. Similar to the in vivo labelling experiments, a 20.5-kDa protein was also more intensely labelled in extracts from salt-shocked cells using [gamma-P-32]ATP in in vitro labelling experiments. Furthermore, labelling of this and a protein of about 64.4 kDa was induced by adding high amounts of NaCl directly to extracts of control cells in kinase assays. Therefore, changes in the NaCl contents led to alterations of the activity of protein kinases in Synechocystis sp. PCC 6803, which may be involved in the signal transduction chain sensing changes in the NaCl content of the surrounding medium.

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收藏

页码：205 / 210

页数：6

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