GLYCOLIPIDS OF A HUMAN GLIOMA CELL-LINE BEARING RECEPTORS FOR PLATELET-DERIVED GROWTH-FACTOR (PDGF)

Glycolipids of U-1242 MG were characterized because results of previous studies showed that exogenous gangliosides, especially G(M3), inhibit PDGF-stimulated growth of this human glioma cell line. G(M3) and G(M2) are the major gangHosides; both separate as doublets with thin-layer chromatography. The major neutral glycolipid is glucocerebroside with nonhydroxy fatty acids, but paragloboside, ceramide dihexoside, globoside, and asialoG(M2) (G(A2)) are also present. The coexistence in U-1242 MG of these gangliosides and the PDGF receptor, whose mitogenic signal is modulated by G(M3) in these cells, suggests a possible functional relationship among them with respect to growth regulation.