THE GENERAL-CHARACTERISTICS OF A BIRNAVIRUS ISOLATED FROM CULTURED LOACH (MISGURNUS-ANGUILLICAUDATUS) IN TAIWAN

被引：7

作者：

CHOU, HY

LO, CF

TUNG, MC

WANG, CH

FUKUDA, H

SANO, T

机构：

[1] NATL TAIWAN UNIV, DEPT ZOOL, TAIPEI, TAIWAN

[2] NATL PINGTUNG POLYTECH INST, DEPT VET MED, PINGTUNG, TAIWAN

[3] FU JEN CATHOLIC UNIV, DEPT BIOL, TAIPEI, TAIWAN

[4] TOKYO UNIV FISHERIES, AQUAT PATHOL LAB, MINATO KU, TOKYO 108, JAPAN

来源：

FISH PATHOLOGY | 1993年 / 28卷 / 01期

关键词：

D O I：

10.3147/jsfp.28.1

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

A birnavirus was isolated from cultured loach (Misgurnus anguillicaudatus) in Taiwan with TO-2 cell line. Evident cytopathic effects of the virus, including karyopycnosis, cytoplasmic inclusion bodies and cell destruction, were observed in TO-2 cells by light microscopy. The naked icosahedral virion layered by a single capsid with a diameter of 69 +/- 4 nm was observed by electron microscopy. Sometimes, complete virions were found to be enclosed by lysosomes to form cytolysome. The virus propagated in several fish cell lines and its replication cycle was completed in 24 hours at its optimum multiplication temperature of 25-degrees-C. This virus has a two segmented double-stranded RNA genome and 3 structural proteins, showing 1.33 g/cm3 buoyant density in CsCl. The chemico-physical properties of the virus together with the result of cross neutralization test indicated that it is a birnavirus and is closely related to Ab serotype of infectious pancreatic necrosis virus (IPNV).

引用

页码：1 / 7

页数：7

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