IDENTIFICATION OF A UNIQUE ANTIGEN PEPTIDE PRL1 ON BALB/C RL-MALE-1 LEUKEMIA RECOGNIZED BY CYTOTOXIC T-LYMPHOCYTES AND ITS RELATION TO THE AKT ONCOGENE

被引：85

作者：

UENAKA, A

ONO, T

AKISAWA, T

WADA, H

YASUDA, T

NAKAYAMA, E

机构：

[1] OKAYAMA UNIV, SCH MED, DEPT PARASITOL & IMMUNOL, OKAYAMA 700, JAPAN

[2] OKAYAMA UNIV, SCH MED, CTR ADULT DIS, DEPT TUMOR IMMUNOL, OKAYAMA 700, JAPAN

[3] OKAYAMA UNIV, SCH MED, INST CELLULAR & MOLEC BIOL, OKAYAMA 700, JAPAN

[4] SETSUNAN UNIV, FAC PHARMACEUT SCI, DEPT ANALYT CHEM, OSAKA 57301, JAPAN

来源：

JOURNAL OF EXPERIMENTAL MEDICINE | 1994年 / 180卷 / 05期

关键词：

D O I：

10.1084/jem.180.5.1599

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

BALB/c radiation leukemia RL male 1 is an immunogenic tumor. We established bulk and cloned cytotoxic T lymphocyte (CTL) lines from regressor (BALB/c x C57BL/6)F-1 (CB6F(1)) spleen cells that recognized RL male 1 specifically. We then obtained antigen peptide recognized by CTL from RL male 1 by acid extraction. Analysis of the acid extract by reversed-phase high performance liquid chromatography (HPLC) on a semipreparative C18 column revealed that fractions eluted in 23 min (peak a) and 26 min (peak b) showed sensitization activity on the P815 target for specific CTL. On further purification of these fractions by HPLC and direct sequencing by Edman degradation, we identified the CTL-recognizing RL male 1 peptide pRL1a (IPGLPLSL) in peak a and its possible precursor peptide pRL1b (SIIPGLPLSL) in peak b. Sequence homology indicated that these peptides were derived from the 5' untranslated region of c-akt oncogene.

引用

页码：1599 / 1607

页数：9

共 52 条

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