Regulation of intracellular free calcium levels by the cellular prion protein

被引：54

作者：

Whatley, SA

Powell, JF

Politopoulou, G

Campbell, IC

Brammer, MJ

Percy, NS

机构：

[1] Department of Neuroscience, Institute of Psychiatry

来源：

NEUROREPORT | 1995年 / 6卷 / 17期

关键词：

prion protein; antibodies; calcium channels; prion diseases;

D O I：

10.1097/00001756-199511270-00015

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

A rat brain synaptosomal model was used to investigate the possible role of the cellular prion protein (PrP) in the regulation of intracellular free calcium levels ([Ca-i(2+)). Treatment of synaptosomes with bacterially derived recombinant human PrP in the range 20-100 mu g ml(-1) resulted in dose-dependent elevations of [Ca2+](i). These increases were dependent on extracellular calcium and were inhibited by gadolinium chloride, a potent blocker of voltage-sensitive calcium channels. Conversely, when calcium channels were activated by synaptosomal depolarization, treatment with monoclonal antibody to PrP in the range 200-320 ng IgG ml(-1) resulted in a dose-dependent reduction of [Ca-i(2+), which was blocked by competition with PrP preparations. These results indicate that PrP is associated with regulation of intracellular free calcium levels through an interaction with voltage-sensitive calcium channels.

引用

页码：2333 / 2337

页数：5

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