CORRELATION BETWEEN POLY(U) MISREADING AND POLY(DT) TRANSLATION EFFICIENCY IN ESCHERICHIA-COLI CELL-FREE SYSTEMS

被引：9

作者：

POTAPOV, AP ^{[1
]}

GROISMAN, IS ^{[1
]}

ELSKAYA, AV ^{[1
]}

机构：

[1] ACAD SCI UKSSR, INST MOLEC BIOL & GENET, KIEV 252143, UKRAINE, USSR

来源：

BIOCHIMIE | 1990年 / 72卷 / 05期

关键词：

antibiotic; misreading; poly(dT); poly(U); protein biosynthesis;

D O I：

10.1016/0300-9084(90)90030-K

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A positive correlation between poly(U) misreading and efficiency of poly(dT)translation has been revealed in cell-free systems from wild-type E coli and streptomycin-resistant mutants with altered ribosomal protein S12. Different factors promoting misreading of poly(U) such as aminoglycoside antibiotics and Mg2+ ions also stimulate poly(dT) translation. The effect of the antibiotics on poly(U) translation efficiency and misreading as well as on poly(dT) decoding is characterised by the same order: nemycin > kanamycin > streptomycin. S12 mutants ribosomes are less erroneous in poly(U) translation and less efficient in poly(dT) decoding. The data obtained are in good agreement with the hypothesis of stereospecific stabilization of codon-anticodon complexes by the ribosome decoding centre. © 1990.

引用

页码：345 / 349

页数：5

共 20 条

[1] ALTERATION OF RIBOSOMAL-PROTEIN S17 BY MUTATION LINKED TO NEAMINE RESISTANCE IN ESCHERICHIA-COLI .1. GENERAL PROPERTIES OF NEAA MUTANTS [J].

BOLLEN, A ;

CABEZON, T ;

WILDE, MD ;

VILLARROEL, R ;

HERZOG, A .

JOURNAL OF MOLECULAR BIOLOGY, 1975, 99 (04) :795-+

[2] COOPERATIVE CONTROL OF TRANSLATIONAL FIDELITY BY RIBOSOMAL-PROTEINS IN ESCHERICHIA-COLI .3. RAM MUTATION IN STRUCTURAL GENE FOR PROTEIN-S5 (RPX E) [J].

CABEZON, T ;

HERZOG, A ;

DEWILDE, M ;

VILLARROEL, R ;

BOLLEN, A .

MOLECULAR & GENERAL GENETICS, 1976, 144 (01) :59-62

[3] STREPTOMYCIN SUPPRESSION + CODE [J].

DAVIES, J ;

GILBERT, W ;

GORINI, L .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1964, 51 (05) :883-+

[4]

DAVIES J, 1968, J BIOL CHEM, V243, P3312

[5]

DAVIES J, 1965, MOL PHARMACOL, V1, P93

[6] A FURTHER STUDY OF MISREADING OF CODONS INDUCED BY STREPTOMYCIN AND NEOMYCIN USING RIBOPOLYNUCLEOTIDES CONTAINING 2 NUCLEOTIDES IN ALTERNATING SEQUENCE AS TEMPLATES [J].

DAVIES, J ;

JONES, DS ;

KHORANA, HG .

JOURNAL OF MOLECULAR BIOLOGY, 1966, 18 (01) :48-+

[7]

GORINI L, 1970, COLD SPRING HARB SYM, V158, P619

[8] SPEED-ACCURACY RELATIONSHIPS DURING INVITRO AND INVIVO PROTEIN-BIOSYNTHESIS [J].

LAUGHREA, M .

BIOCHIMIE, 1981, 63 (03) :145-168

[9] DENATURED DNA AS A DIRECT TEMPLATE FOR IN VITRO PROTEIN SYNTHESIS [J].

MCCARTHY, BJ ;

HOLLAND, JJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1965, 54 (03) :880-+

[10]

MORGAN AR, 1967, J MOL BIOL, V26, P447

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