A 40 KILODALTON RAT-LIVER NUCLEAR-PROTEIN BINDS SPECIFICALLY TO APOLIPOPROTEIN-B MESSENGER-RNA AROUND THE RNA EDITING SITE

Apolipoprotein (apo) B-48 mRNA is the product of RNA editing which consists of a C→U conversion changing a CAA codon encoding Gin-2153 in apoB-100 mRNA to a UAA stop codon in apoB-48 mRNA. in the adult rat, RNA editing occurs both in the small intestine and the liver. We have studied the ability of rat liver nuclear extracts to bind to synthetic apoB mRNA segments spanning the editing site. Using an RNA gel mobility shift assay, we found the sequence-specific binding of a protein(s) to a 65-nucleotlde apoB-100 mRNA. UV crosslinking followed by T1 ribonuclease digestion and SDS-polyacrylamide gel electrophoresis demonstrated the formation of a 40 kDa protein-RNA complex when 32 apoB-100 mRNA was incubated with a rat liver nuclear extract but not with HeLa nuciear extract. Binding was specific for the sense strand of apoB mRNA, and was not demonstrated with singie-stranded apoB DNA, or antisense apoB RNA. The complex also tailed to form if SDS was present during the UV light exposure. Binding experiments using synthetic apoB mRNAs indicate that the 40 kDa protein would also bind to apoB-48 mRNA but not apoA-I, apoA-IV, apoC-II or apoE mRNA. Experiments using deletion mutants of apoB-100 mRNA indicate efficient binding of wildtype 65-nucleotide (W65), 40-nucleotide (W40) and 26-nucleotide (W26) apoB-100 mRNA segments, but not 10-nucteotide (or smaller) segments of apoB-100 mRNA to the 40 kDa protein. in contrast, two other regions of apoB-i00 mRNA, B-5′ (bases 1128-3003) and B-3′ (bases 11310-11390), failed to bind to the protein. The 40 kDa sequence-specific binding protein in rat liver nuclear extract may piay a role in apoB-100 mRNA editing. © 1990 Oxford University Press.