DOMINANT POSITIVE AND NEGATIVE SELECTION USING A HYGROMYCIN PHOSPHOTRANSFERASE-THYMIDINE KINASE FUSION GENE

被引：181

作者：

LUPTON, SD

BRUNTON, LL

KALBERG, VA

OVERELL, RW

机构：

[1] Department of Gene Therapy, Immunex Corporation, Seattle, WA 98101-2977

来源：

MOLECULAR AND CELLULAR BIOLOGY | 1991年 / 11卷 / 06期

关键词：

D O I：

10.1128/MCB.11.6.3374

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The hygromycin phosphotransferase gene was fused in-frame with the herpes simplex virus type 1 thymidine kinase gene. The resulting fusion gene (termed HyTK) confers hygromycin B resistance for dominant positive selection and ganciclovir sensitivity for negative selection and provides a means by which these selectable phenotypes may be expressed and regulated as a single genetic entity.

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页码：3374 / 3378

页数：5

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