FACTORS LIMITING ALIPHATIC CHLOROCARBON DEGRADATION BY NITROSOMONAS-EUROPAEA - COMETABOLIC INACTIVATION OF AMMONIA MONOOXYGENASE AND SUBSTRATE-SPECIFICITY

被引:148
作者
RASCHE, ME [1 ]
HYMAN, MR [1 ]
ARP, DJ [1 ]
机构
[1] OREGON STATE UNIV,DEPT BOT & PLANT PATHOL,NITROGEN FIXAT RES LAB,2082 CORDLEY HALL,CORVALLIS,OR 97331
关键词
D O I
10.1128/AEM.57.10.2986-2994.1991
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The soil nitrifying bacterium Nitrosomonas europaea is capable of degrading trichloroethylene (TCE) and other halogenated hydrocarbons. TCE cometabolism by N. europaea resulted in an irreversible loss of TCE biodegradative capacity, ammonia-oxidizing activity, and ammonia-dependent O2 uptake by the cells. Inactivation was not observed in the presence of allylthiourea, a specific inhibitor of the enzyme ammonia monooxygenase, or under anaerobic conditions, indicating that the TCE-mediated inactivation required ammonia monooxygenase activity. When N. europaea cells were incubated with [C-14]TCE under conditions which allowed turnover of ammonia monooxygenase, a number of cellular proteins were covalently labeled with C-14. Treatment of cells with allythiourea or acetylene prior to incubation with [C-14]TCE prevented incorporation of C-14 into proteins. The ammonia-oxidizing activity of cells inactivated in the presence of TCE could be recovered through a process requiring de novo protein synthesis. In addition to TCE, a series of chlorinated methanes, ethanes, and other ethylenes were screened as substrates for ammonia monooxygenase and for their ability to inactivate the ammonia-oxidizing system of N. europaea. The chlorocarbons could be divided into three classes depending on their biodegradability and inactivating potential: (i) compounds which were not biodegradable by N. europaea and which had no toxic effect on the cells; (ii) compounds which were cooxidized by N. europaea and had little or no toxic effect on the cells; and (iii) compounds which were cooxidized and produced a turnover-dependent inactivation of ammonia oxidation by N. europaea.
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页码:2986 / 2994
页数:9
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