EXPRESSION AND MYRISTOYLATION OF NAP-22 USING A BACULOVIRUS TRANSFER VECTOR SYSTEM

被引：23

作者：

MAEKAWA, S

MATSUURA, Y

NAKAMURA, S

机构：

[1] NATL INST NEUROSCI,NATL CTR NEUROSCI & PSYCHIAT,DIV BIOCHEM & CELLULAR BIOL,KODAIRA,TOKYO 187,JAPAN

[2] NATL INST HLTH,DEPT VIROL 2,SHINJUKU KU,TOKYO 162,JAPAN

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION | 1994年 / 1218卷 / 01期

关键词：

NAP-22; MYRISTOYLATION; BACULOVIRUS; LIPOSOME; CALMODULIN BINDING;

D O I：

10.1016/0167-4781(94)90113-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

NAP-22 is a brain enriched acidic protein localized in the membrane fraction. The peptide sequence of NAP-22 elucidated from the cDNA sequencing, however, showed that NAP-22 is a very hydrophilic protein having no transmembrane regions. The peptide sequence analysis also showed that NAP-22 has a consensus sequence of N-myristoylation and the presence of polybasic domain in its N-terminal region. These sites could be the anchoring sites to localize to the membrane. In this study, we showed the myristoylation of NAP-22 using a Baculovirus expression system and also showed a liposome binding ability of the expressed protein in Escherichia coli.

引用

页码：119 / 122

页数：4

共 14 条

[1]

BLACKSHEAR PJ, 1993, J BIOL CHEM, V268, P1501

[2] MYRISTOYLATED ALPHA-SUBUNITS OF GUANINE NUCLEOTIDE-BINDING REGULATORY PROTEINS [J].